Figure 2 | Scientific Reports

Figure 2

From: Modified cell-permeable JNK inhibitors efficiently prevents islet apoptosis and improves the outcome of islet transplantation

Figure 2

The specificity of 8R-sJNKI(-9). (a) The specificity of 8R-sJNKI(-9). MIN6 cells were cultured with 1 µM of 8R-sJNKI(-9) or 8R-mJNKI(-9) for 23 h. The cells were then treated with 1 µg/mL anisomycin to stimulate JNK activation for 1 h, after which the JNK activity was examined. Western blotting was performed using the rabbit anti-Phospho-c-Jun(Ser73)-specific antibody and mouse anti-β-actin antibody (control). The cell lysates from MIN6 cells treated with and without 1 µg/ml anisomycin for 1 h were used as positive and negative controls, respectively. The data are expressed with the JNK activity of the positive and negative controls, which were arbitrarily set at 100 and 0, respectively. An asterisk indicates a significant difference between the two groups (*p < 0.01). (b) The viability of normal and JNK-inactivated MIN6 cells after exposure of cytokines. MIN6 cells and JNK-inactivated MIN6 cells were cultured with 1 µM 8R-sJNKI(-9) for 23 h followed by exposure to a cocktail of pro-inflammatory cytokines (IL-1β, TNF-α, IFN-γ) for 18 h. Asterisks indicate a significant difference between the two groups (*p < 0.01, ** < 0.05).

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