Figure 4 | Scientific Reports

Figure 4

From: Modified cell-permeable JNK inhibitors efficiently prevents islet apoptosis and improves the outcome of islet transplantation

Figure 4

The function of isolated islets treated with 8R-sJNKI(-9). (a) The number of islets after culture. 2,000 IE of islets were treated with or without 8R-sJNKI(-9) or 8R-mJNKI(-9) for 72 h. After 24, 48, and 72 h of treatment, the cultured islets were counted to calculate the IEs in each group (n = 6). Asterisks indicate significant differences between the no peptide and 8R-sJNKI(-9) groups (*p < 0.01, **p < 0.05). (b) The dose-dependent prevention of the reduction in islet equivalents by 8R-sJNKI(-9). 2,000 IE of islets were incubated for 24 h with 0.05, 0.1, 0.5, or 1 µM of 8R-sJNKI(-9). After 24-h incubation, the islets were counted to calculate the IE in each group. (c) The stimulation index values of the isolated islets. The stimulation index was calculated by determining the ratio of insulin released from the islets in high-glucose media to the insulin released in low-glucose media. The data are expressed as the mean ± SE (each group; n = 6). (d) The cellular ATP content of the isolated islets. The ATP concentration of the cell lysate after preservation was measured using an ATP assay system. The data are expressed as the mean ± SE (each group; n = 6).

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