Figure 5

Transplantation of isolated islets into diabetic mice. Pig islets were treated with or without 1 µM 8R-sJNKI(-9) or 1 µM 8R-mJNKI(-9) for 6 h. A total of 1,500IE of the cultured islets were transplanted below the kidney capsule of immunodeficient diabetic mice. (a) The non-fasting blood glucose levels of mice that were transplanted with islets lacking JNK inhibitor treatment. (b) The non-fasting blood glucose levels of mice transplanted with islets that were treated with the 8R-mJNKI(-9). (c) The non-fasting blood glucose levels of mice transplanted with islets that were treated with the 8R-sJNKI(-9). (d) The percentage of diabetic mice with normoglycemia after islet transplantation. Normoglycemia was defined as two consecutive post-transplant blood glucose measurements of <200 mg/dl. Each group; n = 12 (n = 2 per one isolation) (d) The results of the IPGTT at 30 days after transplantation. The mice were fasted overnight before the test and intraperitoneally injected with glucose (2.0 g/kg body weight). The blood glucose levels were measured before and at 5, 15, 30, 60, and 120 minutes after injection. No peptide and 8R-mJNKI(-9) groups, n = 5 each (diabetic mice after islet transplantation); 8R-sJNKI(-9) group, n = 5 (normoglycemic mice after islet transplantation).