Figure 1
From: Overexpression of Tisochrysis lutea Akd1 identifies a key cold-induced alkenone desaturase enzyme
Cold-induced changes in the alkenone profiles of Emiliania huxleyi and Tisochrysis lutea, and alignment of alkenone desaturase candidates. (a) Partial GC-FID chromatograms of alkenones and alkenoates in the wild type cells of E. huxleyi and T. lutea grown at 25 °C and 15 °C. The figures at the right of arrows are those expanded partially. Peaks: 1, C37:3; 2, C36:3FAME; 3, C37:2; 4, C36:2FAME; 5, C36:2FAEE; 6, C38:3Et; 7, C38:3; 8, C38:2Et; 9, C38:2; 10, C39:3Et, 11, C39:2Et; 12, C39:3; 13, C39:2. Red and blue letters represent alkenones and alkenoates, respectively. (b) Molecular structures of di- and tri-unsaturated C37-alkenones. (c) Alignment of the amino acid sequences of alkenone desaturase candidates. TOD-1 and -2: sequences identified in the T. lutea transcriptome database19. Emihu1_96351, 196284, 444797 and 460273: JGI gene IDs from E. huxleyi. The box indicates amino acid residues conserved in more than four sequences. Blue and yellow: conserved His-boxes and putative trans-membrane regions predicted by the SOSUI engine ver. 1.11 (http://harrier.nagahama-i-bio.ac.jp/sosui/), respectively. Arrow: the initiation of the ORF in the vectors pLF17Akd1-F and -R (Fig. 3a).
