Figure 4
From: Overexpression of Tisochrysis lutea Akd1 identifies a key cold-induced alkenone desaturase enzyme
The maximum likelihood phylogenetic tree and consensus sequences of His boxes in various desaturases. (a) The phylogenetic position of Akd1 annotated as alkenone desaturase among various types of desaturase. *trans-desaturases identified by functional characterization. The scientific name is followed by a simplified regioselectivity of each enzyme (i.e., ∆x is designated as dx). (b) Alignment of consensus sequences of the His boxes in Akd1 and other fatty acid desaturases (revised from Alonso et al.16). X: Arbitrary amino acid residue. Bold and italic: Conserved amino acid residues of histidine and glutamine, respectively. M and P: microsome- and plastid-located enzyme, respectively. (c) Alignment of partial sequences of Akd1-homologues. Black and grey: amino acid residues conserved in all and more than six sequences, respectively. Bar: His box. AP and non-AP represent alkenone producer and non-alkenone producing haptophytes, respectively.
