Figure 7 | Scientific Reports

Figure 7

From: Activation of mineralocorticoid receptor by ecdysone, an adaptogenic and anabolic ecdysteroid, promotes glomerular injury and proteinuria involving overactive GSK3β pathway signaling

Figure 7

MR signaling pathway is required for the albuminuric and glomerulopathic effects of ecdysone. (A) Mice were treated as described in Fig. 6C. On day 0, 7, 14 after various treatments, spot urine was collected and analyzed by mouse albumin ELISA kit and urine creatinine assay kit. Quantification of urine albumin levels adjusted with urine creatinine concentrations; ***P < 0.001 compared with control group and ecdy + spiro group (n = 5). (B) Kidney tissues were procured on day 14 and processed for PAS staining for light microscopy (Bar = 20 µm) or for electron microscopy (Bar = 2 µm). (C) Absolute count of the number of foot processes per unit length of glomerular basement membrane (GBM) on electron micrographs of kidney specimens. **P < 0.01 vs control group, *P < 0.05 vs ecdy + spiro group (n = 5).

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