Table 1 Kinetic parameters of PfCCT enzyme constructs.

From: Structural determinants of the catalytic mechanism of Plasmodium CCT, a key enzyme of malaria lipid biosynthesis

PfCCT

kcat (s−1)

KM,CTP (µM)

KM,ChoP (µM)

581–775

0.010 ± 0.001

1,100 ± 200

N.D.

528–795a

1.45 ± 0.05

170 ± 20

1,950 ± 100

528–795 (K663A)b

0.0008 ± 0.0002

70 ± 20

N.D.

528–795 (Y626F/Q636A)

0.24 ± 0.01

170 ± 10

1,600 ± 100

528–795 (T761A)

0.0010 ± 0.0001

1,270 ± 90

3,700 ± 400

528–795 (T762A)

0.0010 ± 0.0001

610 ± 70

2,100 ± 200

  1. aExperimental data are from Nagy et al.16.
  2. bThe kinetic constant of PfCCT(528–795) K663A refer to a phosphohydrolase activity (see Supplementary Fig. S4).
  3. Values are the mean (±SD) of at least 2 independent experiments.
  4. N.D.: not determined.
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