Figure 5
From: Mechanical loading induces primary cilia disassembly in tendon cells via TGFβ and HDAC6
Cilia disassembly induced by cyclic strain is not caused by changes in actin tension. (a) Representative confocal images showing F-actin in unstrained isolated human tenocytes and following a 24 hour period of cyclic tensile strain (3%, 1 Hz) with and without treatment with 10μM Blebbistatin. Actin labelled with Alexa-Phalloidin. Scale bars represent 50 μm. (b) Corresponding cilia prevalence (values represent mean with error bars indicating standard error for n = 4 fields for each condition). (c) Box and whisker plots showing corresponding cilia length for each condition (N = 40 ± 20 cilia per condition from 1 donor). Data were analysed with 2-factor Anova for both length and prevalence. Significance was observed at the 0.001 level between strain and no strain. However, no significance was observed between blebbistatin and no blebbistatin nor was any significance noted between the interactions. Post hoc testing was performed using Tukey-Kramer. In all cases significance is indicated at p < 0.05 (*), p < 0.01 (**) and p < 0.001 (***).
