Figure 1
From: Neutralization of Junín virus by single domain antibodies targeted against the nucleoprotein
VHHs 1.2, 1.69 and 2.96 neutralize Candid#1 virions and bind to Candid#1 infected cells. (a) The 50% inhibitory concentration (IC50) of the purified VHHs was determined by infection of Vero cells with 50 PFU of Candid#1 that had been previously incubated with serial dilutions of the indicated purified His-tagged VHHs. At 3 days p.i. virus plaques were visualized and counted by immunostaining. Virus yield in the Y-axis is expressed as the percentage of plaques relative to the untreated control. The IC50 value (right) of the VHHs was calculated from three independent experiments (performed in duplicate) and error bars represent the SD of the mean. (b) The binding of the VHHs to infected cells was evaluated by ELISA in Vero cells infected with 50 PFU of Candid#1. At 72 h p.i, cells were fixed, permeabilized and afterwards incubated with serial dilutions of the indicated VHHs (left) or JUNV mAbs (right). (c) Alternatively, Candid#1 infected Vero cells were first incubated with serial dilutions of VHHs or JUNV mAbs and afterwards fixed, without prior permeabilization. VHHs binding curves are depicted in the left panels and N- and GPC-specific mAbs binding curves on the right. Results show the mean of three independent experiments and error bars represent the SD of the mean.
