Figure 6
From: Neutralization of Junín virus by single domain antibodies targeted against the nucleoprotein
VHHs 1.2, 1.69 and 2.96 Candid#1 escape viruses carry a point mutation in GPC. In vitro escape mutants were generated by 3 passages of Candid#1 in the presence of 1000-fold the IC50 of the VHHs. (a) After the passages, escape viruses were evaluated in a neutralization assay by using 50 PFU of the respective escape viruses and 1 µM of VHHs or GPC- and N-specific mAbs. Neutralization was determined by ELISA. Results are expressed as percentage relative to the untreated control. The mean of three independent experiments (n = 3) is shown, where the error bars represent the standard deviation of the mean (SD). (b) Multiple alignment of the predicted amino acid sequence of part of the GPC from the escape mutants obtained under selective pressure with the neutralizing VHHs is shown from residues 141 to 220. The black triangle indicates the altered position in GPC of the escape viruses.
