Figure 2
Prolonged treatment with MPP+ inhibited calcium release and influx in MSC derived dopaminergic neurons. Differentiated MSCs were exposed to 0 (A) or 500 µM MPP+ for 2 (B), 4 (C), 6 (D), 12 (E) or 24 (F) hours. Analog plots of fluorescence ratios (340/380) measured in the presence of 2 µM Thapsigargin, without (first peak of analog plot) or with (second peak of analog plot) 1 mM Ca2+ were determined. A graphical representation of 340/380 ratios (mean ± SE for each group) is represented in (G) and the area under the trace are shown in (H). *p value < 0.05, as compared with differentiated MSCs that were not treated with MPP+ from 50−100 cells in each conditions.
