Figure 3
Prolonged treatment with MPP+ inhibited calcium currents in MSC-derived dopaminergic neurons. Ionositol 1, 4, 5-triphosphate (IP3) (10 µM) induced currents were measured in cells treated with 0 (A) or 500 µM MPP+ for 2 (B), 6 (C), 12 (D) or 24 (E) hours. Addition of SKF 96365 (50 µM) blocked the SOCE currents without changing the properties (A). The holding potential for current recordings was −80 mV. Relative current-voltage (I–V) curves (developed from maximum currents from 6–10 cells in each conditions) and expressed as mean ± SE for each group, are graphically represented in (F). *p value < 0.05, as compared with differentiated MSCs that were not treated with MPP+.
