Figure 1
From: AAV9-mediated Rbm24 overexpression induces fibrosis in the mouse heart
AAV9 injections in wildtype C57/Bl6 mice. (A) Experimental set-up. (B) qPCR analysis of Rbm24 in hearts of AAV9 injected mice, 2 weeks after injection (n = 3–4 per group). (C) Western blot of Rbm24 in hearts of AAV9 injected mice, 2 weeks after injection. (D) Immunohistochemistry of AAV9-induced Rbm24 with an anti-flag antibody (red). Sections were counterstained for α-actinin (green). Nuclei were stained with DAPI (blue). Inset: AAV9-infected cells were α-actinin positive, indicating that AAV9 infected cells are cardiomyocytes (white arrows). Sections were derived from hearts of AAV9 injected mice 2 weeks after injection. Magnification 40x. (E) Percentage of Flag-Rbm24 positive cardiomyocytes in the hearts of AAV9-infected mice.
