Figure 1
From: Temperature responsiveness of gilthead sea bream bone; an in vitro and in vivo approach
Expression of osteogenic genes in gilthead sea bream differentiating bone-derived primary cell cultures in vitro. Quantitative gene expression relative to the geometric mean of RPS18 and EF1α for (A) Runx2, (B) Fib1a, (C) BMP2, (D) Col1A1, (E) ON, (F) OP, (G) MGP and (H) TNAP in cells cultured in growth (GM) or osteogenic (OM) media at days 5, 10, 15 and 20. The results are shown as the mean ± s.e.m. (n = 5–8). Different letters indicate significant differences throughout time within groups (upper case for GM and lower case for OM) and asterisks indicate significant differences between groups at each culture day (p < 0.05). Int.: Interaction, D.C.: Days in culture, Med.: Media.
