Figure 2
From: Temperature responsiveness of gilthead sea bream bone; an in vitro and in vivo approach
Heat map representing changes in osteogenic genes expression in gilthead sea bream bone-derived primary cell cultures incubated at different temperatures. Quantitative gene expression was relative to the geometric mean of RPL27 and Ub for MGP, TNAP, BMP2, Fib1a, OP, HSP30, HSP90b and ON. Changes in gene expression were determined in cells after 13 days growing in osteogenic medium (OM) and incubated at two different temperatures (18 and 28 °C) and normalized in relation to the control temperature of 23 °C at 1, 6, 24 and 48 h (n = 5–8). Rows (mRNA transcripts) in the heat map were standardized following a standard score normalization. Red and green shading, respectively, indicate the highest and lowest expression levels, as specified in the scale bar at the bottom of the figure. Each block represents the average standard-score normalization for the 5–8 cultures sampled at each time-point.
