Figure 3
From: Temperature responsiveness of gilthead sea bream bone; an in vitro and in vivo approach
Expression of osteogenic genes in gilthead sea bream embryos and larvae reared at two different temperatures. Quantitative expression relative to RPS18 of (A) Fib1a, (B) BMP2, (C) Col1A1, (D) ON, (E) OP, (F) MGP, (G) OCN and (H) TNAP at different developmental stages (1 somite [1S.], n = 6; 10 somites [10 S.], n = 6; 25 somites [25S.], n = 6; hatch [H.], n = 6; mouth opening [M.O.], n = 6; early flexion [E.F.], n = 2) in fish reared at two temperatures (18 [LT] and 22 °C [HT]). Results are shown as the mean ± s.e.m. Different letters indicate significant differences through time within groups (upper case for LT and lower case for HT) and asterisks indicate significant differences between groups at each developmental stage (p < 0.05). Although it should be taken into account that only 2 samples (each corresponding to a pool of larvae) were available for the developmental stage of early flexion. n.d.: Non-detected, Int.: Interaction, D.S.: Developmental stage, Temp.: Temperature.
