Figure 4
From: Temperature responsiveness of gilthead sea bream bone; an in vitro and in vivo approach
Expression of osteogenic genes in gilthead sea bream juveniles after a temperature challenge in vivo. Quantitative expression relative to RPL27 of (A) Fib1a, (B) BMP2, (C) Col1A1, (D) ON, (E) OP and (F) OCN. Quantitative PCR was performed with samples from gilthead sea bream reared at different temperature regimes during embryogenesis (LT [18–18 °C]; LHT [18–22 °C]; HT [22–22 °C]; HLT [22–18 °C]) and then maintained at 23 °C (LT n = 9, LHT n = 10, HT n = 9, HLT n = 9) or challenged with a drop in water temperature from 23 °C to 13 °C (LT n = 7, LHT n = 9, HT n = 9, HLT n = 9) at 7 months’ post-hatch. Results are shown as the mean ± s.e.m. No significant differences were observed among thermal regimes within each temperature treatment (23 or 13 °C), but asterisks indicate significant differences between temperature treatments for each thermal regime (p < 0.05). Int.: Interaction, T.R.: Thermal regime, Temp.: Temperature. Variation in sample number for different experimental groups was due to the low yield and quality of RNA extracted from some samples.
