Table 1 Primers used for real-time quantitative PCR.
From: Temperature responsiveness of gilthead sea bream bone; an in vitro and in vivo approach
Gene | Primer sequence (5′-3′) | Tm (°C) | Acc. Num. | Efficiency (%) |
|---|---|---|---|---|
BMP2 | F: GGAGAAGCAGCGTGGATTAAACACGAAT | 68 | AY500244 | 103.9 |
R: GGCCTGCGCCTCAGTCCAAACATATT | ||||
Col1A1 | F: GAGATGGCGGTGATGTGGCGGAGTC | 68 | DQ324363 | 91.7 |
R: GCCTGGTTTGGCTGGATGAAGAGGG | ||||
EF1α | F: CTTCAACGCTCAGGTCATCAT | 60 | AF184170 | 94.2 |
R: GCACAGCGAAACGACCAAGGGGA | ||||
Fib1a | F: CGGTAATAACTACAGAATCGGTGAG | 60 | FG262933 | 101.8 |
R: CGCATTTGAACTCGCCCTTG | ||||
HSP30 | F: GGTGACTGACGGGAAAGAGA | 60 | GU060312 | 93.1 |
R: CTGAGGAGGAGGTGCTGTTC | ||||
HSP90b | F: TTCACGCATGGAAGAAGTTG | 56 | DQ012949 | 86.0 |
R: GGTCCACCACACAACATGAA | ||||
MGP | F: TGTGTAATTTATGTAGTTGTTCTGTGGCATCTCC | 68 | AY065652 | 89.2 |
R: CGGGCGGATAGTGTGAAAAATGGTTAGTG | ||||
OCN | F: TCCGCAGTGGTGAGACAGAAG | 60 | AF048703 | 90.7 |
R: CGGTCCGTAGTAGGCCGTGTAG | ||||
ON | F: AGGAGGAGGTCATCGTGGAAGAGCC | 68 | AY239014 | 97.1 |
R: GTGGTGGTTCAGGCAGGGATTCTCA | ||||
OP | F: AAAACCCAGGAGATAAACTCAAGACAACCCA | 68 | AY651247 | 95.3 |
R: AGAACCGTGGCAAAGAGCAGAACGAA | ||||
RPL27 | F: AAGAGGAACACAACTCACTGCCCCAC | 68 | AY188520 | 97.4 |
R: GCTTGCCTTTGCCCAGAACTTTGTAG | ||||
RPS18 | F: AGGGTGTTGGCAGACGTTAC | 60 | AM490061 | 100.3 |
R: CTTCTGCCTGTTGAGGAACC | ||||
Runx2 | F: ACCCGTCCTACCTGAGTCC | 60 | JX232063 | 96.1 |
R: AGAAGAACCTGGCAATCGTC | ||||
TNAP | F: CATCGCAACCCTTTTCACAGTCACCCG | 68 | AY266359 | 103.2 |
R: AACAGTGCCCAAACAGTGGTCCCATTAGC | ||||
Ub | F: CGGAAGTAAGAGGAACCAACAC | 56 | AM955423 | 78.4 |
R: AAGCAGTCAGAATGCAAAGTCA |
