Figure 3 | Scientific Reports

Figure 3

From: ICAM1 expression is induced by proinflammatory cytokines and associated with TLS formation in aggressive breast cancer subtypes

Figure 3

Expression of ICAM1 in human epithelial cells and breast cancer cells lines. (a) ICAM1 gene expression in breast cancer cell lines compared to a normal breast tissue cell line. (b) The expression of ICAM1 after stimulation of MCF7 cells with different concentrations of the proinflammatory cytokines IFNγ and TNFα were measured by qPCR. (c) Stimulation of TNFα induced the gene expression of ICAM1 in the breast cancer cell lines SK-BR-3, MCF7 and MDA-MB-468 and to a lesser extent in HMLE cells. (d) Stimulation by IFNγ. (e) Stimulation by IL1β. (f) Stimulation by IFNα. (G) Western blot analysis of ICAM1 protein levels in cell extracts from HMLE, SK-BR-3, MCF7 and MDA-MB-468 after stimulation with TNFα, IFNγ, Il1β and IFNα for 24 and 48 hours. Actin is shown as loading control. Full-length blots are available in Supplementary Figs 1–4 Two-way ANOVA, **p < 0.01, ***p < 0.001.

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