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Figure 1

From: Altered secretion patterns and cell wall organization caused by loss of PodB function in the filamentous fungus Aspergillus nidulans

Figure 1

Effect of Ts podB1 mutation on growth and protein productivity. (A) The biomass time course for A28 and ASH83 strains grown in YGV media at 28 °C for 12 h before shifting the temperature to 42 °C. The shift in temperature is shown by the vertical dotted line at 12 h. Temperature shift does not impact the growth of A28 but the biomass of ASH83 does not increase after the temperature is shifted to 42 °C. (B) Specific protein productivity of strains at both 28 °C and 42 °C at 36 h in liquid culture. Abbreviations: A28 grown only at 28 °C (A28@PT), ASH83 grown only at 28 °C (ASH83@PT), A28 grown at 28 °C before shifting to growth at 42 °C (A28@RT) and ASH83 grown at 28 °C before shifting to growth at 42 °C (ASH83@RT). There are no statistically significant differences between A28@PT, ASH83@PT and A28@RT. However, ASH83@RT shows 15X higher protein productivity compared to the other conditions. (n = 3, *p > 0.05). (C) Specific enzyme activity assays (cellulase, white bars; xylanase, grey bars) under same conditions in. (B) There were statistically no significant differences in the specific activities of A28@PT, ASH83@PT and A28@RT. But ASH83@RT had significantly higher specific cellulase and xylanase activity compared to the other conditions (n = 3).

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