Figure 3 | Scientific Reports

Figure 3

From: Tdp-25 Routing to Autophagy and Proteasome Ameliorates its Aggregation in Amyotrophic Lateral Sclerosis Target Cells

Figure 3

Degradative systems involvement in NSC34 cells with GFP-TDPs species. NSC34 transiently transfected with GFP-TDP-43, GFP-TDP-35 and GFP-TDP-25 were treated for 10 hrs with MG132 (10 µM) or for 36 hrs with Wortmannin (50 nM). (a,c) Panel shows NP-40 soluble extracts WB analysis (upper inset, 12% polyacrylamide gels) (full-length blots/gels are presented in Supplementary Figure S7), NP-40 soluble extracts FRA analysis (middle inset) (full-length blots/gels are presented in Supplementary Figure S7) and quantification of NP-40 soluble extracts FRA assay (*p < 0.05; **p < 0,01; ***p < 0.001) (lower inset); (b,d) Panel shows NP-40 insoluble extracts WB analysis (upper inset, 12% polyacrylamide gels) (full-length blots/gels are presented in Supplementary Figure S7), NP-40 insoluble extracts FRA analysis (middle inset) (full-length blots/gels are presented in Supplementary Figure S7) and quantification of NP-40 insoluble extracts FRA assay (*p < 0.05) (lower inset).

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