Figure 1

Palatal phenotypes of K14-Cre/Runx1^fl/fl mice. (A–F) Occlusal views of control and K14-Cre/Runx1^fl/fl mouse palate. An anterior cleft palate was evident at the boundary between the primary and secondary palate and at the first rugae area of the secondary palate in the Runx1 mutant palate at both P50 and P0 (B,D, red arrowheads). Palatal phonotype was not evident at E15.0 (E,F). Scale bar: 1000 μm, P50; 500 μm, P0 and E15.0. (G) Frequency of anterior cleft of control and Runx1 mutant mice at P0. (H) Diagram of the palate shows the position of the frontal section for panels (I–N). The upper sections (I,J) are from the area indicated by the red line (H), the middle one (K,L) by blue line, and the lower ones (M,N) by the black line. (I–N) Histological sections at E17.0 revealed the failure in palatal fusion in the Runx1 mutant shelves (J,L, arrowheads). Scale bar: 200 μm. pp, primary palate; sp, secondary palate; ns, nasal septum; vo, vomeronasal organ; if, incisive foramen; 1St, 1st rugae; 2nd, 2nd rugae.