Figure 6
From: Runx1-Stat3-Tgfb3 signaling network regulating the anterior palatal development
Application of Stat3 inhibitors leading to cleft palate in culture. (A) A Western blot analysis showed that the application of Stat3 inhibitors of Ag490 or S3I-201 suppressed immunoreactivity to pStat3 but not to Stat3. (B) The incidence of cleft palate by Stat3 inhibitors. (C–H) Occlusal views of the palates treated with Stat3 inhibitors. Both Ag490 and S3I-201 impaired the palatal fusion, while the BSA-treated control group showed complete fusion of the palate. The boxed region in the diagram was magnified to evaluate the cleft formation (D) if, incisal foramen. (I–Q) A qPCR analysis of the palatal tissues treated by AG490 or S3I-201. A qPCR analysis showed that application of Stat3 inhibitors disturbed the expression of Tgfb3 and Mmp13 and enhanced the Socs3 expression in a dose-dependent manner.
