Figure 7 | Scientific Reports

Figure 7

From: A Novel Compound, “FA-1” Isolated from Prunus mume, Protects Human Bronchial Epithelial Cells and Keratinocytes from Cigarette Smoke Extract-Induced Damage

Figure 7

FA-1 protects against cigarette smoke-induced cytotoxicity and DNA damage in normal human epidermal keratinocytes. (A) NHEK MTT (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide)-based cell viability after treatment with FA-1 (150 nM in DMSO (dimethyl sulfoxide)) in the presence or absence of 0, 2, 4, and 6% CSE for 24 h. Data are expressed as mean ± SEM (**p < 0.01) cytotoxicity in cultured NHEK; (B) NHEK cells were treated with 150 nM of FA-1 in the presence or absence of 2% CSE for 24 h. Immunoblot analysis was performed for phosphorylation of ATM and H2AX.

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