Figure 2

Temporal shifts in global gene expression upon ciprofloxacin exposure in Neisseria gonorrhoeae. (a) The distribution of −log₂(C:T ratios) for a susceptible isolate (Sus) and resistant isolate (Res) at 0, 5, 10, and 15 min. (b) The fold change in gene expression between control and treated samples (C:T ratio) versus expression in the control sample at 0, 5, 10, and 15 min for one susceptible isolate and one resistant isolate. Genes with C:T ratios above or below the significance threshold are identified as differentially expressed (blue: downregulated; orange: upregulated). Thresholds for statistical significance of fold change (gray lines) are determined by fitting a negative exponential curve (with 90% confidence interval) to the outer edge of the −log₂ C:T ratios measured at time zero (see Methods).