Figure 8
KCNQ4 and KCNMA1 expression in Tg[PTmc1::Tmc2]; Tmc1∆/∆ hair cells. (A) KCNQ4 immunoreactivity38 in Tg[PTmc1::Tmc2]; Tmc1∆/∆ (n = 3 mice), wild-type (Tmc1+/+, n = 3 mice), or Tmc1∆/∆ (n = 3 mice) outer HCs in the apical cochlear turn at P25 (apical turn). KCNQ4 was detected around the perimeter of outer HCs in Tg[PTmc1::Tmc2]; Tmc1∆/∆ mice as well as in wild-type mice, while it was not detected in Tmc1∆/∆ mice. Hair cells were counter-stained with parvalbumin (red). (B) KCNMA1 immunoreactivity38 in Tg[PTmc1::Tmc2]; Tmc1∆/∆ (n = 3 mice each at P16 and P25), wild-type (Tmc1+/+, n = 3 mice each at P16 and P25), or Tmc1∆/∆ (n = 2 mice each at P16 and P25) inner HCs in the apical cochlear turn at P16 and P25. KCNMA1 was detected as typical punctate staining at the neck of inner HCs in wild-type mice at P16 and P25. In Tg[PTmc1::Tmc2]; Tmc1∆/∆ mice, KCNMA1 was detected at P16, but not at P25. In Tmc1∆/∆ mice, KCNMA1 was not detected at P16 or P25. Hair cells were counter-stained with parvalbumin (red; scale bars, 25 µm).
