Figure 2
From: NPM-hMLF1 fusion protein suppresses defects of a Drosophila FTLD model expressing the human FUS gene
Co-expression of NPM-hMLF1 suppressed aberrant eye morphology induced by hFUS. (a) Effects on aberrant eye morphology phenotype induced by hFUS. NE indicates no effect on eye phenotype in adults. Scanning (b,c,e,f,h,i,k,l) and stereoscopic (d,g,j,m) micrographs of adult compound eyes. (b–d) GMR-GAL4 > UAS-GFP (GMR-GAL4/Y; UAS-GFP/+). (e–g) GMR-GAL4 > UAS-NPM-hMLF1 (GMR-GAL4/Y; UAS-NPM-hMLF1/+). (h–j) GMR-GAL4 > UAS-hFUS/UAS-GFP (GMR-GAL4/Y; UAS-hFUS/UAS-GFP). (k–m) GMR-GAL4 > UAS-hFUS/UAS-NPM-hMLF1 (GMR-GAL4/Y; UAS-hFUS/UAS-NPM-hMLF1). (c,f,I,l) Enlarged images of (b,e,h,k) respectively. The eye phenotype of at least five newly eclosed adult male flies of each line was examined and the experiments were done in triplicate. No significant variation in eye phenotype was observed among the five individuals. Anterior is to the left and dorsal to the top. The flies were developed at 28 °C. The bars indicate 50 μm (b,d,e,g,h,j,k,m) and 14.2 μm (c,f,I,l), respectively. White dotted lines define the area of aberrant eye morphology.
