Figure 3
From: NPM-hMLF1 fusion protein suppresses defects of a Drosophila FTLD model expressing the human FUS gene
Specificity of the anti-hMLF1 IgG and immunocytochemical localization of NPM-hMLF1 in eye imaginal disc cells. (a) Western immunoblotting analysis. Proteins were extracted from adult heads carrying GMR-GAL4 > UAS-GFP (GMR-GAL4/Y; UAS-GFP/+) and GMR-GAL4 > UAS-NPM-hMLF1 (GMR-GAL4/Y; UAS-NPM-hMLF1/+). The blots were probed with the anti-hMLF1 IgG. The band with an apparent molecular mass of 56,000 corresponds to NPM-hMLF1 protein. α-tubulin was used as a loading control. The flies were developed at 28 °C. Three independent experiments were carried out. (b–k and b’–i’) Immunostaining of eye imaginal discs with the anti-hMLF1 IgG. (b–e and b’–e’) GMR-GAL4 > UAS-lacZ (GMR-GAL4/Y; UAS-lacZ/+). (f–k and f’–i’) GMR-GAL4 > UAS-NPM-hMLF1 (GMR-GAL4/Y; UAS-NPM-hMLF1/+). Eye imaginal discs were stained with DAPI (b,f,b’ and f’), phalloidin (c,g,c’ and g’) and anti-hMLF1 IgG (d,h,d’ and h’). Merged confocal images are shown in (e,i,j,k,e’ and i’). Higher magnification images of the indicated regions in the panels (b–i) are shown in (b’–i’), respectively. Arrowheads indicate MF. a indicates anterior and p indicates posterior. The flies were developed at 28 °C. Bars indicate 50 μm (b-i) and 5 μm (j,k and b’–i’).
