Figure 8
From: NPM-hMLF1 fusion protein suppresses defects of a Drosophila FTLD model expressing the human FUS gene
Co-expression of NPM-hMLF1 partially rescued the pharate adult lethal phenotype induced by motor neuron-specific expression of hFUS. Assessment of eclosion rate, pupal lethality and larval lethality. First larvae carrying w; UAS-GFP/+; D42-GAL4/+, w; UAS-hFUS/UAS-GFP; D42-GAL4/+ and w; UAS-hFUS/UAS-NPM-hMLF1; D42-GAL4/+ were collected. Ten larvae were reared at 25 °C in a single plastic vial containing fly food. The number of eclosed adult flies, the number of dead pupae that were unable to eclose, and the number of dead larvae that were unable to reach the pupal stage were counted. Eclosed adult flies (48/50), dead pharate adults (2/50) and dead larvae (0/50) were observed in flies carrying w; UAS-GFP/+; D42-GAL4/+ (n = 50). Eclosed adult flies (0/42), dead pharate adults (38/42) and dead larvae (4/42) were observed in flies carrying w; UAS-hFUS/UAS-GFP; D42-GAL4/+ (n = 42). Eclosed adult flies (4/41), dead pharate adults (32/41) and dead larvae (5/41) were observed in flies carrying w; UAS-hFUS/UAS-NPM-hMLF1; D42-GAL4/+ (n = 41).
