Figure 6
MYC67 and MYC70 were able to bind to the promoter region of CBF3/DREB1A. (A) A schematic representation of the promoter region of CBF3/DREB1A and the region that was amplified after the ChIP analysis. (B) The wild-type and transgenic plants used for the ChIP assay were treated at 4 °C for 3 h. The immunoprecipitated DNA with anti-DYKDDDDK tag monoclonal antibody was quantified by quantitative PCR using primers in the promoter region. The data are the average of technical triplicates for the quantitative PCR (mean ± SD). (C) The wild-type and myc mutants were treated at 4 °C for 3 h, and ChIP analysis was performed with anti-ICE1 antibody.
