Figure 3 | Scientific Reports

Figure 3

From: Palmitate-induced lipotoxicity is crucial for the pathogenesis of nonalcoholic fatty liver disease in cooperation with gut-derived endotoxin

Figure 3

Palmitate increased liver inflammation by chemokines via the TLR4 pathway. (A) Cxcl2 and Ccl2 mRNA levels in whole liver were analyzed by qPCR 3 h after palmitate or vehicle injection in BD-fed 8-week-old WT and TLR4 KO mice. (B,C) Immunohistochemical detection of NE and F4/80 (×400) in representative liver samples and average numbers of NE- (×100) and F4/80-positive (×200) cells 3 h after palmitate or vehicle injection were examined in BD-fed 8-week-old WT, TLR4 KO, CXCL2 deficient, CXCR2 KO, CCL2 KO and CCR2 KO mice. n = 5 mice per group. Data represent the mean ± SD. *P < 0.05. NS, not significant.

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