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Figure 1

From: Xbra and Smad-1 cooperate to activate the transcription of neural repressor ventx1.1 in Xenopus embryos

Figure 1

Ectopic expression of Xbra inhibits neurogenesis in a Ventx1.1-dependent manner in animal cap explants of Xenopus. Ventx1.1 MOs (1 ng) were injected either with or without DNBR and Xbra mRNA at the 1-cell stage and dissected the animal cap at the stage 8. The dissected animal caps were harvested until stages 11 and 24 in L-15 culture medium. The relative gene expressions were analyzed by RT-PCR. (a,b) Xbra increases ventx1.1 expression and reduces the expression of early and late neural genes, including that of FoxD5a, N-CAM, Ngnr, and Otx2 while DNBR reduces ventx1.1 expression and induces the expression of neural genes, FoxD5a, N-CAM, Ngnr, and Otx2. (c,d) ventx1.1 MOs reduces ventx1.1 expression and increases the expression of neural genes, including for FoxD5a, N-CAM, Ngnr, and Otx2. C: PCR reaction without adding cDNA while No RT means PCR reaction without added reverse transcriptase.

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