Figure 4 | Scientific Reports

Figure 4

From: Xbra and Smad-1 cooperate to activate the transcription of neural repressor ventx1.1 in Xenopus embryos

Figure 4

Xbra enhances Smad-1 binding affinity on its cis-acting element (BRE) within the ventx1.1 promoter. To perform ChIP-PCR assays, mRNA was co-injected either with or without Smad-1 at the 1-cell stage and harvested the embryos until stage 11 in 30% MMR. (a,b) The ChIP-PCR assay was performed with the anti-Myc antibody. (c,d) The ChIP-PCR assay performed with the anti-Flag antibody at the stage 11. All bindings were measured by PCR method using specific primers. ventx1.1 (−103 and −180) promoter DNA served as positive control. The ChIP-PCR band intensity was quantified using a densitometer.

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