Figure 4 | Scientific Reports

Figure 4

From: Cloning, characterization and functional analysis of an Alveoline-like protein in the shell of Pinctada fucata

Figure 4

Detection of Alv as a matrix protein. (a) Image of mantle with antisense ssRNA probes. (b) Enlarged image of box in image a. The red arrows indicate hybridization signals. (c) Image of mantle with sense ssRNA probes. OF, outer fold; MF, middle fold; IF, inner fold; and MP, mantle pallial. (d) Western blotting analyses of Alv in a matrix protein. ESM, EDTA-soluble matrix; EISM, EDTA-insoluble matrix; P, prismatic layer; N, nacreous layer. Please refer to Fig. S5a,b to get the primary blot pictures.

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