Figure 1 | Scientific Reports

Figure 1

From: High Dose Vitamin D supplementation alters faecal microbiome and predisposes mice to more severe colitis

Figure 1

The experimental approach. 6-week-old C57BL/6 female mice were fed diets with high (D++), moderate (D+) or no (D−) vitamin D3. (A) After 4 weeks of being fed the respective diets, mice were treated with DSS for 6 days to induce colitis, and then were maintained without DSS and continued their respective vitamin D diet until day 35. All mice underwent colonoscopy at day 6, and were sacrificed at day 7, 14, 21 or 35. An additional colonoscopy was performed on day 14, 21 or 35 when mice were sacrificed. (B) A control group of mice fed the three different diets followed the same protocol but were not treated with DSS and the day 21 timepoint is not included. The experiment was performed twice.

Back to article page