Table 1 Effect of various elicitors derived from Corylus avellana L. cell suspension culture on growth and paclitaxel production of Epicoccum nigrum strain YEF2.
Elicitors (% (v/v)) | Fresh weight (g/L) | Dry weight (g/L) | Extracellular paclitaxel (μg/L) | Intracellular paclitaxel (μg/g) | Intracellular paclitaxel (μg/L) | Total yield of paclitaxel (μg/L) | Extracellular paclitaxel portion (%) |
|---|---|---|---|---|---|---|---|
Control (without elicitor) | 43.3 ± 0.0 | 3.11 ± 0.03 | 18.2 ± 0.6 | 2.88 ± 0.17 | 8.97 ± 0.60 | 27.2 ± 1.0 | 67.0 ± 1.4 |
ACE (7%) | 105.6 ± 2.9 | 6.25 ± 0.04 | 109.7 ± 6.5 | 3.05 ± 0.34 | 19.10 ± 2.22 | 128.8 ± 8.8 | 85.3 ± 0.7 |
ACE (14%) | 137.8 ± 2.2 | 8.07 ± 0.06 | 146.1 ± 7.0 c | 3.33 ± 0.34 | 26.93 ± 2.86 | 173.0 ± 8.35 | 84.5 ± 1.4 |
ACE (28%) | 303.3 ± 1.9 | 11.22 ± 0.13 | 219.8 ± 12.3 | 2.98 ± 0.36 | 33.57 ± 4.40 | 253.4 ± 15. 3 | 86.8 ± 1.2 |
FCE (7%) | 137.8 ± 1.1 | 7.17 ± 0.15 | 161.2 ± 10.4 | 3.79 ± 0.47 | 27.18 ± 3.37 | 188.4 ± 9.9 | 85.5 ± 2.0 |
FCE (14%) | 240.0 ± 3.8 | 8.86 ± 0.22 | 236.6 ± 14.4 | 3.80 ± 0.44 | 33.79 ± 4.31 | 270.4 ± 18. 4 | 87.6 ± 0.89 |
FCE (28%) | 384.4 ± 4.8 | 12.64 ± 0.21 | 274.4 ± 11.0 | 3.18 ± 0.42 | 40.30 ± 5.40 | 314.7 ± 15.2 | 87.3 ± 1.2 |
AMF (7%) | 64.6 ± 2.7 | 4.05 ± 0.05 | 76.4 ± 8.2 | 3.10 ± 0.37 | 12.60 ± 1.67 | 89.0 ± 9.32 | 85.8 ± 1.4 |
AMF (14%) | 72.2 ± 2.2 | 4.33 ± 0.04 | 76.9 ± 8.7 | 3.16 ± 0.36 | 13.72 ± 1.66 | 90.6 ± 10.3 | 84.9 ± 0.7 |
AMF (28%) | 102.2 ± 4.8 | 6.28 ± 0.59 | 96.7 ± 3.4 | 3.08 ± 0.39 | 19.81 ± 4.22 | 116.5 ± 7.5 | 83.3 ± 2.6 |
FMF (7%) | 51.1 ± 1.1 | 3.06 ± 0.01 | 224.6 ± 11.9 | 3.23 ± 0.40 | 9.90 ± 1.22 | 234.5 ± 13.07 | 95.8 ± 0.3 |
FMF (14%) | 60.0 ± 1.9 | 3.58 ± 0.04 | 225.3 ± 8.9 | 3.09 ± 0.43 | 11.12 ± 1.66 | 236.4 ± 10.4 | 95.3 ± 0.5 |
FMF (28%) | 66.7 ± 3.3 | 4.05 ± 0.03 | 227.7 ± 9.8 | 3.22 ± 0.36 | 13.10 ± 1.59 | 240.8 ± 11.3 | 94.6 ± 0.4 |
