Figure 7

OsCYCB1;1 gene and pCYCB1;1::GUS expression in rice seedlings. Seedlings were grown in hydroponic medium containing normal nutrition (control; 2.5 mM N, 300 μM P), low N, or low P (LN, 0.02 mM; LP, 2 µM), or subjected to treatment with sodium nitroprusside (SNP, 10 µM) or GR24 (analog of SLs, 2.5 µM) for 14 days. (a) The expression of OsCYCB1;1 gene in WT, d10 and d3 mutants. (b) pCYCB1;1::GUS expression in WT and ospin1b-1 mutant. Cell cycle activity of the root meristem, as monitored by the pCYCB1;1::GUS reporter, is shown. Bar = 500 μm. Data are means ± SE of three replicates (a) and bars with different letters indicate significant differences at p < 0.05, as determined by ANOVA followed by the LSD test.