Figure 2 | Scientific Reports

Figure 2

From: Improved phosphoproteomic analysis for phosphosignaling and active-kinome profiling in Matrigel-embedded spheroids and patient-derived organoids

Figure 2

Results of phosphoproteomics from 3D HCT116 cultures embedded in Matrigel. (A) A bar graph of identified number of class 1 phosphosites from Matrigel-embedded HCT116 spheroid in protocol with centrifugation (a black bar) or acetone precipitation (white bars: samples collected with the D method, gray bars: samples collected with the CR method). (B) Venn diagrams of phosphosites identified with the D method (left panel) and the CR method (right panel). (C) Number of submitted MS/MS spectra (white bars) and MS/MS spectra identifying peptides (black bars). N = 3. Error bars indicate standard deviations.

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