Figure 4 | Scientific Reports

Figure 4

From: Improved phosphoproteomic analysis for phosphosignaling and active-kinome profiling in Matrigel-embedded spheroids and patient-derived organoids

Figure 4

Comparison of phosphosites identified from 2D- and 3D-cultured HCT116 cells. (A) Proportional Venn diagram of phosphosites identified in all triplicate experiments from 2D and 3D culture of HCT116 cells. (B) Volcano plot of phosphosites identified in all triplicate experiments from 2D-cultured HCT116 cells and 3D cultured HCT116 spheroids. Magenta circles: increased phosphosites in the samples in the D or the CR method relative to 2D-cultured samples, Cyan circles: decreased phosphosites in the samples in the C or the CR method relative to 2D-cultured samples, Gray circles: phosphosites without significant differences. (C) Workflow for the selection of phosphosites that were significantly increased or identified only in the 3D-cultured samples.

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