Table 1 Deduced amino acid sequences of engineered HACS motifs of reverse genetics viruses.

From: Evolution of high pathogenicity of H5 avian influenza virus: haemagglutinin cleavage site selection of reverse-genetics mutants during passage in chickens

Reverse genetics virus

HACS motif a

HA1

HA2

P12

P11

P10

P9

P8

P7

P6

P5

P4

P3

P2

P1

P1’

rgHPAIV

N

S

P

Q

R

E

R

R

R

K

K

R

G

rgLPAIV

N

S

P

Q

R

E

—

—

—

—

T

R

G

Mutant 5

N

S

P

Q

R

E

—

—

—

—

K

R

G

Mutant 6

N

S

P

Q

R

—

—

—

—

K

T

R

G

Mutant 7

N

S

P

Q

—

—

—

—

R

K

K

R

G

Mutant 4

N

S

P

Q

R

E

—

—

R

K

K

R

G

Mutant 8

N

S

P

Q

—

—

—

R

R

K

K

R

G

Mutant 9

N

S

P

Q

R

E

—

R

R

K

K

R

G

Mutant 2

N

S

P

Q

R

E

R

R

R

K

T

R

G

Mutant 1

N

S

P

Q

R

E

R

R

Q

K

K

R

G

Mutant 3

N

V

P

Q

R

E

R

R

R

K

K

R

G

  1. 1Dash indicates deletion, underlined indicates mutation. Cleavage occurs C-terminally at the scissile bond between P1 (HA1) and P1’ (HA2). The HACS motif is flanked N-terminally by PQ, and C-terminally by G.
Back to article page