Figure 1 | Scientific Reports

Figure 1

From: Na/K-ATPase Y260 Phosphorylation–mediated Src Regulation in Control of Aerobic Glycolysis and Tumor Growth

Figure 1

Identification of Y260 in α1 Na/K-ATPase as the major Src binding site- (a) Comparison of Y260 containing sequences in second cytoplasmic domain (CD2) of different human Na/K-ATPase isoforms. (b) Interaction between CD2 and Src in different cell lines. Representative blots are shown, n = 3. (c) Effects of ouabain on ERK activation. *p < 0.05 compared with vehicle-treated control of the same cell line (Student’s T-test). #p < 0.05 compared between different cell lines (One-way ANOVA). n = 4. (d) Detection of Y260 phosphorylation in CD2. Representative blots are shown, n = 3. (e) Y260 phosphorylation and expression of α1 Na/K-ATPase in mouse tissues. A representative blot is shown, n = 3.

Back to article page