Figure 6
Colonization of CEABC10 transgenic (Tg) mice deficient in Eif2ak4 gene with AIEC LF82 results in modification of the gut microbiota composition at days 14 and 21 post-infection. Tg/eif2ak4+/+ and Tg/eif2ak4−/− mice were challenged by oral gavage for 3 days (once per day) with PBS (N = 6 mice per group) or with 109 CFU of the AIEC LF82 strain (N = 6 mice per group). Feces were collected at days 14 (A–D) and 21 (E–H) post-infection for the analysis of the bacterial microbiota composition based on Illumina sequencing of the 16S rRNA gene. (A–H) Mouse fecal bacterial communities were clustered using PCoA of the weighted UniFrac distance matrix. PCoA-1 and PCoA-2 were plotted, and the percentage of the variation explained by the plotted principal coordinates was indicated in the Y-X-axis labels. Groups were compared using permanova method. Ns, not significant; *P < 0.05; **P ≤ 0.01. (A,E) Comparison between mouse groups: Tg/eif2ak4+/+ + PBS, Tg/eif2ak4+/+ + LF82, Tg/eif2ak4−/− + PBS and Tg/eif2ak4−/− + LF82. (B,F) Comparison between mouse groups: Tg/eif2ak4+/+ + PBS and Tg/eif2ak4+/+ + LF82. (C,G) Comparison between mouse groups: Tg/eif2ak4−/− + PBS and Tg/eif2ak4−/− + LF82. (D,H) Comparison between mouse groups: Tg/eif2ak4+/+ + LF82 and Tg/eif2ak4−/− + LF82.
