Figure 9
Expression of A. fumigatus Prxs is regulated by sakAHOG1 and yap1YAP1 in the presence of paraquat and fludioxonil. (A,B) mRNA abundance of prx1, prxB and prxC is impacted by the MAP kinase SakA while the expression of prx1 and prxC is regulated by the Yap1 transcription factor. Wild-type, ΔsakA, ΔmpkC and Δyap1 mutant strains were grown in MM for 24 h and subjected to treatment with fludioxonil (1 µg/ml; A) or paraquat (10 mM; B) for 15, 30 or 60 minutes. The control was left untreated. The mRNA abundance for each gene was assessed by RT-qPCR and normalized to β-tubulin. The data represent the average value of three biological replicates, each of which was repeated in duplicate in the same run, Bar = SD, *p ≤ 0.05 (one-way ANOVA). (C,D) The Prx deletion mutants are more sensitive to fludioxonil and iprodione. 1 × 104 conidia of each strain were grown on MM containing increasing concentrations of fungicides. The cultures were grown for 72 h at 37 °C. The results are representative of four independent experiments.
