Figure 2 | Scientific Reports

Figure 2

From: Clonal expansion of mtDNA deletions: different disease models assessed by digital droplet PCR in single muscle cells

Figure 2

Comparison between ddPCR and Southern blot results on muscle homogenate or blood DNA. Southern blot indicated mtDNA deletion levels of 76.0% and 74.0% in DNA extracted from muscle homogenates of patients 3 and 6, respectively. Mean values from ddPCR were 73.1% (SD = 2.3) and 53.0% (SD = 4), respectively. In patients 22 and 23, heteroplasmy rate of mtDNA deletions on Southern blot in blood derived DNA was 70.0% and 50.0%, while ddPCR estimated 63.7% (SD = 0.58) and 55.7% (SD = 3.2), respectively. Heteroplasmy rate of mtDNA deletions in muscle homogenate DNA from controls 1 and 2 validated by ddPCR was 1.0% (SD = 0.8) and 0.9% (SD = 0.8), respectively. All ddPCR experiments were performed in triplicates. The SD values are presented as error bars.

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