Table 2 Primers and probes used in ddPCR experiments.

From: Clonal expansion of mtDNA deletions: different disease models assessed by digital droplet PCR in single muscle cells

ND1 Forward

5′-CCCTAAAACCCGCCACATCT-3′

ND1 Reverse

5′-GAGCGATGGTGAGAGCTAAGGT-3′

ND1 Probe

5′-FAM/CCATCACCCTCTACATCACCGCCC/BHQ1-3′

ND4 Forward

5′-CCATTCTCCTCCTATCCCTCAAC-3′

ND4 Reverse

5′-CACAATCTGATGTTTTGGTTAAACTATATTT-3′

ND4 Probe

5′-HEX/CCGACATCATTACCGGGTTTTCCTCTTG/BHQ2-3′

  1. Primer numbers refer to the mtDNA Genbank reference sequence NC 012920.1.
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