Figure 6

Evaluation of tolerogenic dendritic cells (tDCs). Spleens from 14-week-old male and female WT (n = 10), ERAP1^−/− (n = 12), and CF ERAP1^−/− (n = 8) mice were harvested, processed, stained and analyzed by flow cytometry. (a) Relative frequencies of tolerogenic CD45RB^high CD11c^low dendritic cells (tDCs) in WT, ERAP1^−/−, and CF ERAP1^−/− spleens plotted on scatter plot bar graph, with representative images in (b). (c) Graph of relative frequencies of Qa-2 expressing F4/80 + macrophages. (d) Graph, depicting percent of Qa-2 expressing CD45RB^high CD11c^low tDCs in WT, ERAP1^−/−, and CF ERAP1^−/− mice. (e) Representative histogram images demonstrating relative abundance of Qa-2 positive tDCs. (f) Graph depicting Mean Fluorescence Intensity (MFI) of surface Qa-2 on tDCs from WT, ERAP1^−/−, and CF ERAP1^−/− mice. Statistical significance was determined using One Way ANOVA, and Tukey post hoc test. Data are representative of two independent experiments, with similar results.