Figure 2

CRB2 immunofluorescence identification in the nervous system. Double immunofluorescence for CRB2 (A,B,D) and GFAP (A,C,D) in coronal sections of adult mouse PVN, showing that the two proteins do not colocalize in any of the cells or profiles. Double immunofluorescence labeling for CRB2 (E,G,H,J) and Iba1 (F,G) or Olig1 (I,J) in coronal sections of adult mouse PVN, showing that CRB2 is not present in microglia or oligodendrocytes. DAPI (in blue): Nuclear labeling. Scale bars: 100 µm (A), 15 µm (B–D), 20 µm (E–J).