Figure 1
From: Isolation and characterisation of alveolar type II pneumocytes from adult bovine lung
Schematic of the purification procedure from animal to culture. (a) Lungs were obtained directly from slaughter and taken back to the laboratory, where aseptic dissection took place. (b) The tissue was then washed repeatedly with DPBS containing EDTA and penicillin/streptomycin. (c) Tissue was digested at 37 °C, then filtered sequentially through mesh of decreasing pore sizes. (d) The filtrate was overlaid onto bovine IgG-coated bacterial grade petri dishes to remove fibroblasts and macrophages. (e) Non-adherent cells were then loaded in 4% Percoll™ onto a Percoll™ gradient, overlaying onto 10–30% Percoll, which generated an ATII enriched emulsion at the 10–30% interface. (f) These cells were then washed, counted and plated out in SAGM, containing 100 U/mL penicillin/streptomycin.
