Figure 6
From: Isolation and characterisation of alveolar type II pneumocytes from adult bovine lung
Removal of contaminating fibroblasts from cultures of isolated ATII epithelial cells. (a) (i) Fibroblasts, residual from the isolation process, are highlighted by arrows. Cells subjected to HBSS treatment for a minimum of 30 minutes, result in an adherent and non-adherent phenotype. (ii) Cobblestone morphology exhibited by adherent cells, indicative of epithelial cells. (iii) Non-adherent cells with a spindle-like morphology, indicative of fibroblast enrichment. (b) IF staining with the ATII marker Pro SP-C and co-stain with CD90, a surface marker previously identified in both epithelial1 and fibroblast41 cultures; adherent cells are positive for both markers, whilst non-adherent cells appear negative for Pro SP-C expression. Images representative of three treatments, each of 30 minutes duration.
