Table 1 Theoretical and experimental values of the protein radius, a, height, hc, and hydrodynamic area, Ahydro(0).

From: Hydrodynamic trapping measures the interaction between membrane-associated molecules

 

SA

CD2

CD4

CD45

Theorya

a [nm]

2.8

1.5

1.5

1.5

hc [nm]

5

7.5

11

>15

Ahydro(0) [nm2]

296

299

513

≥820

Experimentb

Ahydro(0) [nm2]

300 ± 29

324 ± 18

505 ± 40

1536 ± 352

hc [nm]c

5.1 ± 0.4

8.0 ± 0.3

10.9 ± 0.6

22.2 ± 3.1

ahd [nm]d

3.2 ± 0.4

5.3 ± 0.2

3.7 ± 0.3

8.0 ± 0.3e

  1. aTheory from: Darst et al. (SA)4, Davis and van der Merwe (CD2)20, Yin et al. (CD4)8, and Chang et al. (CD45)7.
  2. bExperimental values are given as mean ± SD.
  3. cEstimated height value (mean ± SD) calculated from Supplementary equations (S9) and (S10) using the experimental Ahydro(0) and the theoretical a.
  4. dFit of μex to a hard-disk model, with ahd being the disk radius.
  5. eValue at c = 500 molecules/μm2. ahd = 5.3 ± 0.1 nm at c = 6000 molecules/μm2.
Back to article page